Description
The Real-time PCR System YR06248//YR06249 is a cutting-edge tool designed for precision and accuracy in qPCR applications. With innovative signal gathering and spectrum separation technology paired with unique temperature control capabilities, this PCR system stands out in terms of detection sensitivity and temperature uniformity. Offered in both 4-channel and 5-channel configurations, it supports a wide range of qPCR dye-based and probe-based chemistries, making multiplex PCR applications effortless. Standard analysis methods like absolute quantification, genotyping, HRM, MMCA, and gene expression analysis are seamlessly integrated into this system.
Market Price
The real-time PCR systems are a significant investment in any laboratory setting. Typically, the market price for high-quality systems like the YR06248//YR06249 ranges from approximately 23,500 USD to 23,800 USD. This pricing reflects the advanced technology and reliability that these systems bring to molecular biology laboratories. For an exact quotation tailored to your specific needs, feel free to inquire or generate an automatic quote via the Kalstein Plus platform.
Frequently Asked Questions
What are the main differences between the 4-channel and 5-channel models? The primary difference lies in the number of detection channels available, which allows for more complex multiplexing capabilities with the 5-channel model.
Is the system compatible with all qPCR dyes? The system is designed to support a wide spectrum of dyes, including FAM/SYBR Green, VIC/JOE/HEX/TET, ROX/Texas Red, and Cy5/LIZ, with optional support for Cy5.5 in the 5-channel model.
Advantages and Disadvantages
One of the main advantages of the Real-time PCR System YR06248//YR06249 is its excellent reproducibility and high resolution, allowing for precise quantification over a wide dynamic range. The system’s innovative optic design eliminates multi-color crosstalk, enhancing reliability in results. However, one potential downside could be the initial cost of investment, which might be substantial for smaller labs.
Usage in the Field
The Real-time PCR System YR06248//YR06249 is extensively used in research laboratories for gene expression analysis, genotyping, and pathogen detection. Its fast and precise thermal cycling feature is crucial in fields demanding rapid turnaround times, such as clinical diagnostics and environmental monitoring.
Recommendations
To maximize the system’s efficiency, regularly calibrate the system as per manufacturer guidelines and ensure periodic maintenance. Leveraging the system’s multiplexing capabilities can significantly reduce time and reagent costs in the lab environment. For optimal use and support, turning to Kalstein Plus for service and updates is highly recommended.
Features
- Fast and Precise Thermal Cycling
- Innovative Optic Design
- Excellent Reproducibility
- Up to 10-log Dynamic Range
- Melting Curve Analysis
- High Resolution
Technical Specifications
| Model | YR06248 | YR06249 |
| Sample capacity | 8*0.1mL PCR strip*12 96*0.1mL individual tube 0.1mL PCR Plate |
|
| Sample volume | 10-50 μL | |
| Heating and Cooling method | Peltier | |
| Maximum ramping rate | 6.0° C/s | |
| Temperature range | 4-100 °C | |
| Temperature accuracy | ± 0.2°C | |
| Temperature uniformity | ±0.2°C@60°C ±0.3°C@95°C | |
| Optical Detecting System | ||
| Excitation light | 4pcs high-efficiency single color LED | 5pcs high-efficiency single color LED |
| Detector | SiPMT | |
| Detection method | Time-resolved real-time scanning | |
| Excitation/Emission wavelengths | 455-650nm | 510-750nm |
| Detection Channels | 4 (optional 5 channels） | |
| Supported dyes | FAM/SYBR Green, VIC/JOE/HEX/TET, ROX/Texas Red, Cy5/LIZ，（Optional- Cy5.5 for 5-channel model） | |
| Multiplexing | Up to 4 targets (optional 5 targets for 5-channel model) | |
| Sensitivity | 1 copy gene | |
| Resolution | 1.33-fold copies difference in single-plex reaction | |
| Dynamic range | 10 orders of magnitude | |
